For daily calculations, DPRA-ZymaX uses standards of known value relative to the international standards. For example, for organics, NBS-22 (light oil standard; δ¹⁸O = -29.8‰, δD = -120‰). Below is a list of all daily standards used at DPRA-ZymaX. These standards are collected with samples on the gas separation vacuum line in the ratio of 1 standard:10 samples or 1 standard per work order: whichever is smaller.

Standards

Sample duplicates are collected in a ratio of 1 duplicate:10 samples. Line blanks are also determined on a regular basis.

Isotopic compositions of standards of known composition are determined at the mass spectrometer on a daily basis prior to sample collection in order to verify running conditions.

The accuracy of software calculations in the measurement and correction of isotope ratios is monitored by manual calculations.

Laboratory technicians are thoroughly trained in both the theory and application for sample preparation and isotope ratio measurement. Personnel files contain listings of approved method training and standard data where applicable.

The accuracy of such measurements can of course be influenced by factors other than the precision of the mass spectrometer - such as sample purity, sample size and operation technique.

Acceptable DPRA-ZymaX sample duplicate reproducibilities are shown below but may vary by sample type and sample size:

δD ± 3.0‰
δ³⁴S ± 0.3‰
δ¹⁸O ± 0.3‰
δ¹⁵N ± 0.3‰
δ¹³C ± 0.2‰
δ³⁷Cl ± 0.5‰